Mohammad Navaderi , farnoosh Gholamhoseinpoor,sharareh Khorami
Farnoosh Gholamhoseinpoor, Green synthesized nanoparticlesZnO Evaluation of Antimicrobial(2015)SDRP Journal of Computational Chemistry & Molecular Modelling 1(1)
Introduction: Introducing a powerful antibacterial agent to control pathogenic bacteria especially strains resistant to antibiotics is of paramount importance. The purpose of this study is ZnO nanoparticles synthesis by using plant extracts ziziphora teniri l and Asteraceae evaluation of its antibacterial properties.
Methods: Zinc oxide nanoparticles were synthesized using plant extracts ziziphora teniri l and Asteraceae. Then physical and chemical properties of nanoparticles were studied. Escherichia coli and Staphylococcus aureus through standard methods such as placing well and disc diffusions, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC).
Results: Chemical and physical investigation of the synthesized ZnO nanoparticles indicated that from nanoscale particles, spherical forms were produced with no impurities. The MIC for E.coli and Staph.aureus were reported as 0.125 mg/ml, and 0.062 mg/ml respectively. The MBC for E.coli and Staph. aureus was reported to be 0.500 mg/ml, and 0.250 mg/ml respectively. In comparison, for the methods of well and disc diffusions, inhibition zone diameter of the well method was more than disc diffusion.
Conclusion: High purity zinc oxide nanoparticles can be synthesized by plant extracts ziziphora teniri l and Asteraceae have antibacterial properties against Escherichia coli and Staphylococcus bacteria. In contrast to Escherichia coli, Staph. aureus showed greater sensitivity against Zinc Oxide nanoparticles.
Keywords: ZnO nanoparticle, Antibacterial, Gram-negative, Gram-positive, synthesized, MIC, Chemical.
• (TEM ) Transmission electron microscope
• (SEM) Scanning electron microscope
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