Maria Aparecida Marin-Morales
Sumitha K V(email@example.com)
Bruna de Campos Ventura Camargo, Micronuclei and chromosome aberrations derived from the action of Atrazine herbicide in Allium cepa meristematic cells(2016)SDRP Journal of Earth Sciences & Environmental Studies 1(1)
Atrazine is an herbicide that causes serious damage to the environment and possibly affects the genetic material of organisms that come into contact with this agent, provoking mutations in their DNA. Although the herbicide Atrazine has been tested by various systems, a large number of deficiencies have been reported for many assays, and some evidence of its genotoxic effects still needs to be confirmed. The objective of the present study was to evaluate the herbicide Atrazine effects, analyzing the mitotic index alterations and the induction of chromosome aberrations, using the Allium cepa test system. Fifty Allium cepa seeds were used for germination in Atrazine solutions at the concentration used in the field (0.125 ppm) and at progressively lower concentrations (0.062, 0.031 and 0.015 ppm). After 20 h, some roots were then collected from each assay, while the remaining roots were transferred to plates containing Milli-Q water and allowed to recover for 48 h. The herbicide reduced the germination indexes of seeds, the mitotic indexes of cells and induced alterations in the genetic material of the test organism, what suggests that this herbicide presents genotoxic effect at all concentrations tested.
Allen, K.J., Lepp, D., McKellar, R.C., Griffiths, M.W., Bolle, P., Mastrangelo, S., Tucci, P., Evandri, M.G., 2004. Clastogenicity of atrazine assessed with the Allium cepa test. Environmental and Molecular Mutagenesis. 43, 137-141. PMid:14991755View Article PubMed/NCBI
Brusick, D., 1987. General Toxicology: Principles of Genetic Toxicology, New York.
Chauhan, L.K.S., Savena, P.M., Gupta, S.K., 1999. Cytogenetics effects of cypermethrin and fenvalerate on the root meristem cells of Allium cepa. Environmental and Experimental Botany. 42, 181-189. 00033-7View Article
El-Ghamery, A., El-Nahas, A.I., Mansour, M.M., 2000. The Action of Atrazine Herbicide as an Inhibitor of Cell Division on Chromosomes and Nucleic Acids Content in Root Meristems of Allium cepa and Vicia faba. Cytologia. 65, 277-287.View Article
Eldridge, J.C., Wetzel, L.T., Stevens, J.T., Simpkins, J.W., 1999. The mammary tumor response in triazine-treated female rats: a threshold-mediated interaction with strain and species-specific reproductive senescence. Steroids. 4, 672-678. 00051-3View Article
Fenton, S.E., Greiner, S.N., Youngblood, G.L., Davis, C.C., 2002. Effects from gestational exposure to a mixture of Atrazine and its biological metabolites in male Long-Evans rats. Biology of Reproduction. 66, 199-200.
Fiskesj?, G., 1985. The Allium test as a standard in environmental monitoring. Hereditas. 102, 99-112. PMid:3988545View Article PubMed/NCBI
Fiskesj?, G., 1993. Technical Methods Section. Allium test I: A 2-3 Day plant test for toxicity assessment by measuring the mean root growth of onions (Allium cepa L.). Environmental Toxicology and Water Quality. 8, 461-470.View Article
Khors, S.S., Panda, K.K., Panda, B.B., 1997. Genotoxicity of tetrodotoxin from buffer fish tested in root meristem cells of Allium cepa. Mutagenesis. 12, 265-269.View Article
Kligerman, A.D., Doerr, C.L., Tennant, A.H., Peng, B., 2000. Cytogenetic studies of three triazine herbicides II. In vivo micronucleus studies in mouse bone marrow. Mutation Research. 471, 107-112. 00124-8View Article
Kovalchuck, O., Kovalchuck, I., Arkhipov, A., Telyuk, P., Hohn, B., 1998. The Allium cepa chromosome aberration test reliably measures genotoxicity of soil of inhabited areas in the Ukraine contaminated by the Chernobyl accident. Mutation Research. 415, 47-57. 00053-9View Article
Leme, D.M., Marin-Morales, M.A. 2009. Allium cepa test in environmental monitoring: a review on its application. Mutation Research. 82, 71-81. PMid:19577002View Article PubMed/NCBI
Ma, T.H., Xu, C., Mcconnell, H., Rabago, E.V., Arreola, G.A., Zhang, H., 1995. The improved Allium/Vicia root tip micronucleous assay for clastogenicity of environmental pollutants. Mutation Research. 334, 185-95. 90010-1View Article
Marin-Morales, M.A., Ventura-Camargo, B.C., Hoshina, M.M., 2013. Toxicity of herbicides: impact on aquatic and soil biota and human health. In: Herbicides, InTech ? Open Access Company, p.399-443 ( ). https://doi.org/10.5772/55851View Article
Marshall, R. J., Nel, P.C., 1980. Study of Atrazine phytotoxicity to sorghum by split root technique. Crop Production. 9, 141-143.
Matsumoto, S.T., Mantovani, M.S., Malagutti, M.I.A., Dias, A.L., Fonseca, I.C., Marin-Morales, M.A., 2006. Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips. Genetics and Molecular Biology. 29, 148-158.View Article
Mazzeo, D.E.M, Marin-Morales, M.A., 2015. Genotoxicity evaluation of environmental pollutants using analysis of nucleolar alterations. Environmental Science and Pollution Research. 22, 9796-9806. PMid:25639248View Article PubMed/NCBI
Popa, N.E., Zakrzhevskaya, A.M., Kozhokaru, R.V., Enaki, D.K., 1986. Cytogenetic effect of some herbicides on maize seedlings. Weed Abstracts. 35, 50.
Rank, J., Nielsen, M.H., 1993. A modified Allium test as a tool in the screening of the genotoxity of complex mistures. Hereditas. 118, 49-53.View Article
Rank, J., Nielsen, M.H., 1997. Allium cepa anaphase-telophase root tip chromosome aberration assay on N-methyl-N-Nitrosourea, maleic hydrazide, sodium azide, and ethyl methanesulfone. Mutation Research. 390, 121-127. 00008-6View Article
Rank, J., Nielsen, M.H., 1998. Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay. Mutation Research. 418, 113-119. 00118-1View Article
Ribas, G., Surrall?s, J., Carbonell, E., Creus, A., Xamena, N., Marcos, R., 1998. Lack of genotoxicity of the herbicide Atrazine in cultured human lymphocytes. Mutation Research. 416, 93-99. 00081-3View Article
Smaka-Kincl, V., Stegnar, P., Loukam, T.M.A.M., 1997. The evaluation of waste, surface and ground water qualify using the Allium test procedure. Mutation Research. 38, 171-179.
Sudhakar, D., Panda, K.K., Panda, B.B., 1998. Biomonitoring of low levels of mercurial derivatives in water and soil by Allium micronucleus assay. Mutation Research. 203, 11-21.
Tennant, A.H., Peng, B., Kligerman, A.D., 2001. Genotoxicity studies of triazine herbicides in vivo studies using the alkaline single gel (SCG) assay. Mutation Research, 493, 1-10. 00145-0View Article
Ventura, B.C., Angelis, D.F., Marin-Morales, M.A., 2008. Mutagenic and genotoxic effects of the Atrazine herbicide in Oreochromis niloticus (Perciformes, Cichlidae) detected by the micronuclei test and the comet assay. Pesticide Biochemistry and Physiology, 90, 42-51.View Article
Ventura-Camargo, B.C. Maltempi, P.P.; Marin-Morales, M.A., 2011. The use of the cytogenetic to identify mechanisms of action of an azo dye in Allium cepa meristematic cells. Journal of Environmental and Analytical Toxicology, 1, 1-12.View Article
Vogel, E.W., 1982. Assessment of chemically-induced genotoxic events: Prospectives and Limitations, Leiden.