Patricia Lucio Alves, Paulo Flavio Silveira, and Rafaela Fadoni Alponti
Rafaela Fadoni Alponti Vendrame, Adipogenesis and reduction of lipid accumulation in retroperitoneal fat pad are stimulated by food deprivation.(2016)SDRP Journal of Cellular and Molecular Physiology 1(1)
This study aimed to test the hypotheses that lipid drop density (dL), lipocrite (Li) and mean diameter (Dm), volume (V) and mass (MAC) of adipocytes provide reliable values of the number of adipocytes in vivo (NA), and that fasting alters NA. Mean diameter (?m) and lipocrite were respectively measured by microscopy and by the ratio between relative volume occupied by isolated adipocytes in a fixed volume suspension obtained from fixed sampled mass of retroperitoneal fat pad (MAT) from rats maintained under normal feeding (NF) or submitted to food deprivation for 72 h (FD). V(mL)=(?Dm3/6).10-12, MAC(g)=dLxV, and NA were calculated by (i) Li/V and (ii) MAT/MAC. Li was higher in FD than in NF, while Dm was lower in FD than in NF. NA, calculated by (i) and (ii), was higher in FD than in NF. Increased Li in FD should be due to hyperplasia, assuming that it does not occur through increased Dm (hypertrophy), since FD had lower Dm than NF. Data suggested that in vivo adipogenesis occurs in retroperitoneal fat pad after 72 h of fasting as an homeostatic response to lipolysis and an adjustment to provide increased energy storage when there is new food supply.