Alaa A. El-Talees, Noha I. Hussein ,Nora Nasr Arafat
SDRP Journal of Cellular and Molecular Physiology
VOLUME: 1 ISSUE: 1
naglaa, THE EFFECTS OF EXERCISE AND ANTIOXIDANT ON KLOTHO GENE EXPRESSION DURING OXIDATIVE STRESS IN RAT
Obesity has been described as a state of chronic oxidative stress. There are several ways in which obesity directly affects the cardiovascular system. The Klotho gene acts as an aging -suppressor gene that protects cells and tissues from oxidative stress. It’s now well known that exercise or antioxidants can prevent oxidative damage by reducing oxidative stress. This study was carried out in order to clarify the effects of exercise and antioxidants on Klotho gene mRNA expression in rat’s cardiac tissue during oxidative stress .The animals were divided into 5 groups. The group I serves as negative control. Group II is a positive control group, feeding high fat diet (HFD)(Oxidative stress group) .Group III: (HFD) treated with exercise training. Group IV: (HFD) treated with vitamin E as antioxidant and group V: (HFD) treated with exercise training and vitamin E. Serum insulin, glucose, HOMA, total cholesterol and TG were measured. Gene expression of TNF? mRNA expression level in cardiac tissue and Klotho mRNA expression in kidney tissue were performed using Real time PCR. We demonstrate that combined exercise training with vitamin E treatment for 8 weeks resulted in a significant improvement in the insulin resistance and dyslipidemia. Also, it was associated with significant decrease in TNF? expression in heart and increase in Klotho gene expression in kidney. We concluded that the exercise training and /or antioxidant treatment improved oxidative stress effects in cardiac tissue through up-regulates mRNA expression of Klotho gene in the kidney and down-regulation of TNF? expression in the heart.